The fuel landscape has steadily been changing and it is expected to evolve at a much rapid pace over the coming years. There will be a shift towards low-carbon and zero-carbon fuels for power generation and transportation. These fuels will initially see increased usage in the form of blending components to conventional fuels and subsequently as stand-alone fuels. In this context, we have been carrying out experimental and modeling work to predict ignition characteristics of alternative fuels. Shock tubes and rapid compression machine are used as homogeneous reactors to measure fuel reactivity and autoignition behavior. Additionally, laser-based measurement and theoretical calculations are employed to target critical elementary reactions that control fuel pyrolysis and oxidation.